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Terminology: TCID50(Median Tissue Culture Infectious Dose)
TCID50 (Median Tissue Culture Infectious Dose)
The TCID50 (Median Tissue Culture Infectious Dose) assay is one method used to verify the viral titer of a testing virus.
Host tissue cells are cultured on a well plate titer, and then varying dilutions of the testing viral fluid are added to the wells.
After incubation, the percentage of infected wells is observed for each dilution, and the results are used to calculate the TCID50 value.