Terminology: Rat Acute Inhalation Toxicity Test

The objective of this test is to examine the harmful effects caused by the test substance when inhaled into lungs through respiration, such as direct damage to respiratory organs and consequential general damage to the body caused by the absorption of a test substance through the lungs.

[Test Method]
A test was conducted with rats as test animals. Five each of male and female rats were placed in a chamber in which the atmosphere was conditioned to contain 2.86mg/l of Zeomic powder, causing the rats to inhale the air for 4 hours.
Then they were kept for 4 days as per the EPA OPPTS 870.1300 method.

[Test Results for Zeomic]
As a result, not even a single fatal case was observed in spite of the use of 2.86mg/l, the upper limit of concentration specified in the test protocol. The anatomy of the animals after the test confirmed that no anomalies had developed in any of their organs.
As a result, acute inhalation toxicity LC50 was determined at 2.86mg/l.

Pertaining to Rat Acute Inhalation Toxicity Test

Contact us

To top