- Zeomic and antimicrobial-related terminology
- Antimicrobial agent and antimicrobial test terminology
- Virus terminology
- Odor-eliminating test terminology
- Test terminology
- Approval/license and certification terminology
Terminology: Chronic Toxicity Test
This test is to examine the quantity of a test substance producing some kind of toxic effect and the quantity of a test substance producing no effect by observing changes appearing in a test animal's morphological features, when it is administered repeatedly to a test animal for a long period.
The objective of this test is to examine the quantity of a test substance producing some kind of toxic effect (observed adverse effect level) and the quantity of a test substance producing no effect (no observed adverse effect level) by observing changes appearing in a test animal's functions and morphological features, when it is administered repeatedly to a test animal for a long period (12 months or longer). The test reported here is a particularly exhaustive one based on in-depth analysis from the testing of the organs of the test animals and as far-reaching as to the blood, which is considered to meet quite a high standard even from a medical research point of view.
A test was conducted with rats and mice as test animals, which were divided into groups consisting of 1000 animals each, and feed containing Zeomic was orally fed everyday for a period determined for each group, the longest of which was 2 years, which corresponded to the entire life of the test animals.
[Test Results for Zeomic]
As a result, no changes that could be histopathologically regarded as a problem, including carcinogenicity, were found to have developed in the organs. The no-observed effect level (NOEL) in the chronic toxicity test was determined at 0.011g/kg-bw/day. Calculated from these figures, it was determined that the acceptable daily intake (ADI) for Zeomic is 0.00011g/kg-bw/day, which is a comparable level to existing food additives in Japan today.
The acceptable daily intake of some chemical compounds approved as food additives in Japan are as follows: dibutylhydroxyltoluene: 0.0005g/kg-bw/day, saccharine: 0.0025g/kg-bw/day, thiabendazole: 0.00005g/kg-bw/day. No carcinogenicity or cancer facilitative properties were found.